Neuritogenic and antioxidant activities of nordentatin from Clausena harmandiana

Author: Pongsakorn Jantakoon, Sarin Tadtong, Ploenthip Puthongking

Category: Pharmacy

Abstract:

Nordentatin, a member of coumarins from natural sources, was identified from root bark of Clausena harmandiana and previously tested by 1,1-diphenyl-2- pricrylhydrazyl (DPPH) and thiobarbituric acid reactive substances (TBARS) indicating its potent antioxidant and inhibition of lipid peroxidation. This lead to further isolate of its derivatives, dentatin and xanthoxyletin, and evaluate their activities on neuritogenic and neurotoxic using cultured P19 neurons and antioxidant effects. Nordentatin showed no neurotoxicity on the cultured cells (IC50>10 __ampersandsignmu;M) while dentatin and xanthoxyletin expressed neurotoxicity at 1 nM (viability 62.28---PlusMinusSymbol--- 12.11% and 43.02---PlusMinusSymbol---10.31%, respectively). Phasecontrast micrographs showed that nordentatin promoted neurites outgrowth of the neurons. The neurons treated with nordentatin displayed significantly more branching numbers of the neurites than the control (p < 0.05). It can be concluded that nordentatin is a remarkable neuritogen from natural product.

Keywords: antioxidant activity Clausena harmandiana coumarins neuritogenicity nordentatin