ISSN 2286-6493
Journal of Asian Association of Schools of Pharmacy
 

Genotoxicity assessment of recombinant human interferon gamma in human lymphocytes  Research Paper 

Sepideh Arbabi Bidgoli1, Masomeh Heshmati1,2, Heydeh Keyhan1 and Atefeh Afshary3
1 Department of Toxicology and Pharmacology, Islamic Azad University.
2 Department of Molecular Biology, Islamic Azad University.
3 Department of Pharmaceutical Registration, Exir Pharmaceutical Company, Tehran, Iran
 

Abstract

    Interferon (IFN)-gamma as a recombinant pharmaceutics, being launched in Iran as the third country in the world, despite its unclear genotoxic potential in in vivo models. In the present study, the genotoxic effects of this recombinant human IFNgamma were evaluated in peripheral blood lymphocytes from 24 healthy volunteers. The induction of chromosomal alterations including chromosomal aberrations, and micronuclei (MN) in binucleated cells were performed by different cytogenetic assays. Heparinized whole-blood samples from volunteers after cell pretreatment were treated with 0.33-66.6 IU/μl of IFN-gamma. The treated cells were then determined for chromosomal aberrations, and micronuclei in binucleated cells. The negative and positive controls were phosphate buffered saline (PBS), and mitomycin C, respectively. Results revealed that cells treated with IFN showed no significant difference from cells treated with PBS indicating this recombinant protein under the performed treatment regimen lacked of genotoxic potential.  



Keywords

1 genotoxicity
2 recombinant interferon
3 IFN-gamma
4 chromosome aberration
5 micronuclei



Published in

JAASP Volume 1 No. 2
April - June, 2012

Page: 107-115


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