ISSN 2286-6493
Journal of Asian Association of Schools of Pharmacy

Assessment of mutagenic and genotoxic activity of Caesalpinia sappan L. extract by Ames test and micronucleus assay  Research Paper 

Pranom Puchadapirom1, Lakana Himakoun1,Yuvadee Wongkrajang2 Rungravi Temsiririrkkul2, Penchom Peungvicha2 and Boontium Kongsaktrakoon2
1 Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
2 Department of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
3 Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand


    The mutagenic activity of the water extract of sappan heartwood (Caesalpinia sappan L.) was evaluated by Ames test. A plate incorporation method with and without the rat liver mixture (S9 mix) using Salmonella typhimurium strains TA98 and TA100 as indicator strains was applied in the present study. This plant extract exhibited an antibacterial potential at the concentration above 2.0 mg/plate either with or without S9 mix. However, it showed no mutagenic activity at the concentration between 0.25 and 2.5 mg/plate. The genotoxicity of the extract from sappan heartwood was further assayed by a cytokinesis – block micronucleus test using Chinese Hamster lung fibroblast (V79 cell line). It was found that the extract showed the cytotoxic effect to V 79 cell line at 400 and 200 μg/ml in the presence and absence of S9 mix, respectively, but was revealed to have no toxic effect to DNA observed at the concentration between 5 and 200 μg/ml.  


1 Caesalpinia sappan
2 genotoxic activity
3 mutagenic activity
4 Ames test
5 micronucleus assay

Published in

JAASP Volume 1 No. 2
April - June, 2012

Page: 97-106

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