ISSN 2286-6493
Journal of Asian Association of Schools of Pharmacy
 

Neuritogenic and antioxidant activities of nordentatin from Clausena harmandiana  Research Article 

Pongsakorn Jantakoon1, Sarin Tadtong2 and Ploenthip Puthongking1
1 Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
2 Faculty of Pharmacy, Srinakharinwirot University, Nakhon nayok , 26120, Thailand.
 

Abstract

    Nordentatin, a member of coumarins from natural sources, was identified from root bark of Clausena harmandiana and previously tested by 1,1-diphenyl-2-pricrylhydrazyl (DPPH) and thiobarbituric acid reactive substances (TBARS) indicating its potent antioxidant and inhibition of lipid peroxidation. This lead to further isolate of its derivatives, dentatin and xanthoxyletin, and evaluate their activities on neuritogenic and neurotoxic using cultured P19 neurons and antioxidant effects. Nordentatin showed no neurotoxicity on the cultured cells (IC50>10 ?M) while dentatin and xanthoxyletin expressed neurotoxicity at 1 nM (viability 62.28? 12.11% and 43.02?10.31%, respectively). Phase-contrast micrographs showed that nordentatin promoted neurites outgrowth of the neurons. The neurons treated with nordentatin displayed signifi-cantly more branching numbers of the neurites than the control (p < 0.05). It can be concluded that nordentatin is a remarkable neuritogen from natural product.  



Keywords

1 antioxidant activity
2 Clausena harmandiana
3 coumarins
4 neuritogenicity
5 nordentatin



Published in

JAASP Volume 1 No. 3
July - September, 2012

Page: 180-186


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