ISSN 2286-6493
Journal of Asian Association of Schools of Pharmacy
 

Analgesic, anti-inflammatory, antipyretic activities and acute toxicity of the ethanolic extract of Clausena harmandiana Pierre in animals  Research Article 

Jinda Wangboonskul1, Auemduan Prawan2, Pobporn Takthaisong3, Watikan Sasithornwetchakun3, Chantana Boonyarat3, Chavi Yenjai4 and Pramote Mahakunakorn3
1 Faculty of Pharmacy, Thammasat University, Pathum Thani, 12120, Thailand.
2 Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
3 Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
4 Faculty of Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand
 

Abstract

    Clausena harmandiana Pierre (CH), a local plant used traditionally for pain relief and antipyretic, was used to investigate analgesic, anti-inflammatory, antipyretic activities and acute toxicity from an ethanolic extract of its root bark (CH-EtOH). Chemical analysis by HPLC indicated dentatin and nordentatin contents in CH-EtOH at 1.71 and 2.57% w/w, respectively. Analgesic activity of oral CH-EtOH was tested in mice by thermal threshold tests and acetic acid (AA)-induced writhing response, antiinflammatory by carrageenan-induced rat paw edema, antipyretic by baker yeast-induced fever rat model and acute toxicity followed OECD Guidelines. Oral CH-EtOH significantly decreased writhing responses in a dose-dependent manner (p<0.05), but slightly affected thermal thres-hold, suggesting its peripheral analgesic action. It showed no antiinflammatory activity but significantly reduced the rectal temperature on yeast-induced pyrexia (p<0.05) in a dose-dependent manner comparable to paracetamol. Acute toxicity test of oral CH-EtOH up to 3 g/kg did not show any serious toxic effects during a 7-day period of observations. This evidenced the use of ethanolic extract of root bark of C. harmandiana in folk medicine as analgesic and antipyretic agents.  



Keywords

1 Clausena harmandiana
2 analgesic
3 anti-inflammatory
4 antipyretic
5 acute toxicity
6 folk medicine



Published in

JAASP Volume 1 No. 3
July - September, 2012

Page: 159-169


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