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<ArticleSet>
  <Article>
    <Journal>
      <PublisherName>aaspjournal</PublisherName>
      <JournalTitle>Journal of Asian Association of Schools of Pharmacy</JournalTitle>
      <PISSN>I</PISSN>
      <EISSN>S</EISSN>
      <Volume-Issue>Volume 2</Volume-Issue>
      <PartNumber/>
      <IssueTopic>Multidisciplinary</IssueTopic>
      <IssueLanguage>English</IssueLanguage>
      <Season>January - December, 2013</Season>
      <SpecialIssue>N</SpecialIssue>
      <SupplementaryIssue>N</SupplementaryIssue>
      <IssueOA>Y</IssueOA>
      <PubDate>
        <Year>-0001</Year>
        <Month>11</Month>
        <Day>30</Day>
      </PubDate>
      <ArticleType>Pharmacy</ArticleType>
      <ArticleTitle>Iron chelating activity of Pyoverdin PaII and pseudobactin 589 siderophores with plasma iron</ArticleTitle>
      <SubTitle/>
      <ArticleLanguage>English</ArticleLanguage>
      <ArticleOA>Y</ArticleOA>
      <FirstPage>269</FirstPage>
      <LastPage>279</LastPage>
      <AuthorList>
        <Author>
          <FirstName>Saengrawee</FirstName>
          <LastName>Sutthiparinyanont</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>N</CorrespondingAuthor>
          <ORCID/>
          <FirstName>Chalerm</FirstName>
          <LastName>Ruangviriyachai</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
        </Author>
      </AuthorList>
      <DOI/>
      <Abstract>Two kinds of siderophores produced from two bacterial species, Pseudomonas aeruginosa and P. putida, called pyoverdin PaII (pvd-PaII) and pseudobactin 589 (psdb-589), respectively. Both of them were investigated for their iron scavenging effects upon plasma iron and compared with commercial iron chelator named esferoxamine B (DFO-B) by malondialdehyde-2-thiobarbituric acid assay (MDA-TBA assay). These siderophores were produced by the optimized conditions using 6% sucrose, 0.4% L-asparagine and K2HPO4 with the incubation temperature at 25?C. They were isolated and purified by Amberlite XAD-4 and Bio-Gel P-2 columns. Each of siderphore-iron complexes was at the ratio of 1:1. The content of iron in plasma sample determined by atomic absorption spectrophotometer was in the range of 0.81- 1.68 ?g/ml. The iron scavenge was represented by the reduction of lipid peroxidation in plasma measured by MDATBA content. At the concentration of 35.8 ?M of 3 iron chelating agents, DFO-B, pvd-PaII and psdb589, could reduce the average lipid peroxidation in plasma (n = 6) by 11.2, 27.0 and 29.4%, respectively. The reduction of plasma MDA compared between pvd-PaII and psdb-589 or pvdPaII and DFO-B was not significantly different (p &gt; 0.05). It can be concluded that the biological iron chelator of siderophore especially psdb-589 reveals capability to reduce iron which is the cause of lipid peroxidation in plasma.</Abstract>
      <AbstractLanguage>English</AbstractLanguage>
      <Keywords>Siderophore lipid peroxidation MDA-TBA assay</Keywords>
      <URLs>
        <Abstract>https://aaspjournal.org/ubijournal-v1copy/journals/abstract.php?article_id=5941&amp;title=Iron chelating activity of Pyoverdin PaII and pseudobactin 589 siderophores with plasma iron</Abstract>
      </URLs>
      <References>
        <ReferencesarticleTitle>References</ReferencesarticleTitle>
        <ReferencesfirstPage>16</ReferencesfirstPage>
        <ReferenceslastPage>19</ReferenceslastPage>
        <References/>
      </References>
    </Journal>
  </Article>
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