Two kinds of siderophores produced from two bacterial species, Pseudomonas aeruginosa and P. putida, called pyoverdin PaII (pvd-PaII) and pseudobactin 589 (psdb-589), respectively. Both of them were investigated for their iron scavenging effects upon plasma iron and compared with commercial iron chelator named esferoxamine B (DFO-B) by malondialdehyde-2-thiobarbituric acid assay (MDA-TBA assay). These siderophores were produced by the optimized conditions using 6% sucrose, 0.4% L-asparagine and K2HPO4 with the incubation temperature at 25?C. They were isolated and purified by Amberlite XAD-4 and Bio-Gel P-2 columns. Each of siderphore-iron complexes was at the ratio of 1:1. The content of iron in plasma sample determined by atomic absorption spectrophotometer was in the range of 0.81- 1.68 ?g/ml. The iron scavenge was represented by the reduction of lipid peroxidation in plasma measured by MDATBA content. At the concentration of 35.8 ?M of 3 iron chelating agents, DFO-B, pvd-PaII and psdb589, could reduce the average lipid peroxidation in plasma (n = 6) by 11.2, 27.0 and 29.4%, respectively. The reduction of plasma MDA compared between pvd-PaII and psdb-589 or pvdPaII and DFO-B was not significantly different (p > 0.05). It can be concluded that the biological iron chelator of siderophore especially psdb-589 reveals capability to reduce iron which is the cause of lipid peroxidation in plasma.
