Join us   Log in   jaasp@aaspnet.org  


JOURNAL OF ASIAN ASSOCIATION OF SCHOOLS OF PHARMACY - Volume 13 Issue 1, January - December 2024

Pages: 1-5
Print Article   Download XML  Download PDF

Weak electric current increases ceramide levels by inducing ceramide synthase expression

Author: Naoshi Yamazaki, Chiho Ohtsuka, Kentaro Kogure

Category: Pharmacy

Abstract:

We previously demonstrated that weak electric current (WEC) induces the skin penetration of macromolecules by the cleavage of intercellular junctions and allows for incorporation of macromolecules into cells via endocytosis. We further showed that WEC facilitates the leakage of macromolecules from endosomes to cytoplasm and that this phenomenon correlates with increased endosomal ceramide levels. From these observations, it suggested ceramide production is induced by WEC. In this study, to investigate mechanisms by which WEC treatment increases ceramide levels, acyl chains in ceramides of WEC-treated cells were analyzed. As a result, we found that WEC increased the amounts of C16:0, C22:0, and C24:1 ceramides and that WEC induced ceramide synthase 2 expression. Therefore, we conclude that WEC activates the ceramide synthesis pathway and that increased endosomal ceramide would contribute to the leakage of macromolecules.

Keywords: weak electric current, ceramide synthesis, cytoplasmic delivery

DOI: https://doi.org/10.62100/jaasp.2024.13101

DOI URL: https://doi.org/10.62100/jaasp.2024.13101